【文獻標題】 The distinct role of CD73 in the progression of pancreatic cancer
【作者】Liangjing Zhou1,Shengnan Jia1,Yan Chen,et.al
【作者單位】浙江大學(Zhejiang University)
【文獻中引用產品】
【關鍵詞】Pancreatic cancer,Progression,CD73,TNFR2,miR-30a-5p
【DOI】doi.org/10.1007/s00109-018-01742-0
【影響因子(IF)】4.14
【出版期刊】 《Journal of Molecular Medicine》
【產品原文引用】
Detection of TNF-α levels in supernatant via ELISA
Supernatant was investigated for levels of TNF-α protein using commercial TNF-α ELISAs (Hengyuan Biotechnology Co., Ltd., Shanghai, China) according to the manufacturer’s instructions.
Western blot
Cells were harvested, and total protein lysates were resolved using 10% sodium dodecyl sulfate–polyacrylamide gels,electroblotted onto a polyvinylidene fluoride membrane,blocked by 5% skim milk, and probed with primary antibodies listed in Table S1. The membrane was then incubated with the secondary antibody and visualized by enhanced chemiluminescence using Kodak X-Omat LS film (Eastman Kodak,Rochester, USA).
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