国产一区二区三区波多野结衣AV_日韩午夜在线视频_俺去啦最新官网在线_国产无夜激无码AV毛片_日韩午夜精品毛片_国产在线高清无码视频_中文 国产 亚洲 喷潮_2017最新理论电影在线_波多野给衣一区二区三区_成年女人毛片视频免费

您好,歡迎光臨上海恒遠生物科技商城!
全國服務(wù)熱線:18221290082
關(guān)注我們

快速導(dǎo)航

首頁 > 產(chǎn)品文獻

恒遠產(chǎn)品文獻:試劑-藏紅O,固綠FCF引用文獻
發(fā)布時間:2020/7/17 10:53:38 閱讀次數(shù):938

【文獻標(biāo)題】GR/HDAC2/TGFβR1 pathway contributes to prenatal caffeine inducedosteoarthritis susceptibility in male adult offspring rats

【作者】Jing Li,Hao Xiao,Hanwen Luo,et.al

【作者單位】武漢大學(xué)基礎(chǔ)醫(yī)學(xué)院(Wuhan University School of Basic Medical Sciences)

【文獻中引用產(chǎn)品】

試劑-藏紅O

試劑-固綠FCF

【關(guān)鍵詞】Caffeine,Osteoarthritis,Glucocorticoid,Transforming growth factor β receptor 1,Histone modification

【DOI】https://doi.org/10.1016/j.fct.2020.111279

【影響因子(IF)】3.78

出版期刊】《Food and Chemical Toxicology》

【產(chǎn)品原文引用】

Materials

Caffeine (CAS #58-08-2, > 99% purity) was purchased from SigmaAldrich Co., Ltd. (St Louis, MO, USA). Safranin-O (CAS. NO. 477-73-6)and fast green (CAS. NO. 2353-45-9) were obtained from Hengyuan Biotech Co., Ltd. (Shanghai, China). RIPA lysis buffer and BCA Protein Assay kit were purchased from Beyotime Institute of Biotechnology (Haimen, China). An enzyme-linked immunosorbent assay (ELISA) kit for detecting serum corticosterone concentration was purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Reverse transcription and

real-time quantitative polymerase chain reaction (RT-qPCR) kits were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). The oligonucleotide primers for rat RT-qPCR genes (PAGE purification)were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China).Chondroitin sulfate sodium salt from shark cartilage, DMEM/F12 (1:1)and fetal bovine serum (FBS) were supplied by Gibco (St Louis, MO,USA). Polyclonal rat antibodies including TGFβR1 (ab31013), SMAD2/3 (ab232326), SOX9 (ab185230), HDAC2 (ab213700), aggrecan (ab36861) and Col2 (ab34712) were purchased from Abcam plc.(Cambridge, Cambridge shire, UK). The histone deacetylase 2 (HDAC2)siRNA oligo was purchased from Gene Pharma Co. (Jiangsu, China).Other chemicals and agents were of analytical grade.


完整版PDF文獻請咨詢在線客服或者電話聯(lián)系我司業(yè)務(wù)員!

更多公司福利請關(guān)注“恒遠生物”微信公眾號!

首頁|產(chǎn)品專區(qū)|品牌專區(qū)|優(yōu)惠促銷|產(chǎn)品文獻|公司簡介|付款方式

關(guān)注恒遠生物科技

服務(wù)熱線:

18221290082

13636351073

13564766906

13636351217

18221656311

13817140470