【文獻標題】PM2.5 exposure induces reproductive injury through IRE1/JNK/autophagy signaling in male rats 

【作者】Yang Yang，Yajing Feng，Hui Huang，et.al

【作者單位】鄭州大學第一附屬醫(yī)院（The First Affiliated Hospital of Zhengzhou University）

【文獻中引用產品】

大鼠睪酮（T）ELISA試劑盒

【關鍵詞】Fine particulate matter，Inositol-requiring enzyme，C-Jun NH 2-terminal kinase，Autophagy，Reproductive injury

【DOI】https://doi.org/10.1016/j.ecoenv.2021.111924 

【影響因子(IF)】6.20

【出版期刊】《Ecotoxicology and Environmental Safety》

【產品原文引用】

2.5. Determination of serum testosterone 

We evaluated serum testosterone level in rats by ELISA. After the rats were sacrificed, approximately 5 mL of abdominal aortic blood was collected and subjected to centrifugation at 3000 rpm for 15 min, and the serum was carefully aspirated and stored at  20 for later use. According to the instructions for the rat testosterone ELISA kit (Hengyuan Biotech, Shanghai, China), we used different concentrations of testosterone standards and corresponding absorbance values to prepare a standard curve, and thus determined the testosterone in each group of samples after observing the sample absorbance. 

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