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恒遠(yuǎn)產(chǎn)品文獻(xiàn):蚯蚓8-羥基脫氧鳥苷(8-OHdG)ELISA試劑盒引用文獻(xiàn)
發(fā)布時(shí)間:2021/4/19 11:25:19 閱讀次數(shù):779

 【文獻(xiàn)標(biāo)題】Ecotoxicity evaluation of azoxystrobin on Eisenia fetida in different soils

【作者】Yaqi Xu,Bing Li, Kaixuan Hou,et.al
【作者單位】山東大學(xué)( Universities of Shandon)
【文獻(xiàn)中引用產(chǎn)品】
蚯蚓8-羥基脫氧鳥苷(8-OHdG)ELISA試劑盒
【關(guān)鍵詞】Strobilurin fungicide ,Earthworms ,Toxicity evaluation ,Oxidative damage ,Natural soil 
【DOI】https://doi.org/10.1016/j.envres.2020.110705 
【影響因子(IF)】8.4
出版期刊】《Environmental Research》
【產(chǎn)品原文引用】
Reactive oxygen species (ROS) content was determined using the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) method (Guo et al., 2015). 2′,7′-dichlorofluorescein is the product of reaction of ROS and DCFH-DA, which can be detected by a fluorescence spectrophotometer (RF-5301PC, Shimadzu, Japan). MDA content was determined according to Han et al. (2014) by evaluating thiobarbituric acid chromogenic reaction. The UV absorbance were read at 532 nm. DNA oxidative damage indicator (8-OHdG) was determined using enzyme linked immunosorbent assay kit (Shanghai Hengyuan Biological Technology Co., Ltd, China). The samples were read at 450 nm by microplate reader (MK3, Thermo Fisher, USA). 
 

 
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