【文獻(xiàn)標(biāo)題】RPL35A is akey promotor involved in?the?development and progression of gastric cancer
【作者】Fang Wu, Dachuan Sun, Yuqian Liao, et.al
【作者單位】中國人民解放軍總醫(yī)院(Chinese, PLA General Hospital)
【文獻(xiàn)中引用產(chǎn)品】
山羊血清
【關(guān)鍵詞】Gastric cancer, RPL35A, Cell proliferation, Cell apoptosis, Cell migration
【DOI】https://doi.org/10.1186/s12935-021-02199-x
【影響因子(IF)】4.9
【出版期刊】《Cancer Cell International》
【產(chǎn)品原文引用】
Immunohistochemical staining
First, gastric cancer and normal tissue microarrays were collected and dewaxed through washing with xylene and alcohol. Ten the samples were subjected to antigen retrieval with 1 × EDTA (Beyotime Biotechnology Co., Ltd., Shanghai, China) at 100? °C for 30? min. After that, the slides were cooled to room temperature, soaked in 1× PBS bufer and blocked with 3% H2O2 for 5?min and goat serum (Hengyuan Bio Co., Ltd.) for 15? min.
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