【文獻標題】Efficacy confirmation of Scutellaria baicalensis Georgi in the treatment of periodontitis via topical administration and active ingredients screening
【作者】Zhen Huang , Yi Yu , Xiao-Liang Lin , et.al
【作者單位】中國藥科大學(China Pharmaceutical University)
【文獻中引用產(chǎn)品】
大鼠白介素1β (IL-1β)ELISA試劑盒
大鼠白介素6(IL-6)ELISA試劑盒
大鼠白細胞介素8(IL-8)ELISA試劑盒
大鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒
大鼠前列腺素E2(PGE2)ELISA試劑盒
【關(guān)鍵詞】Periodontitis ,Scutellaria radix ,Pharmacodynamic basis ,Traditional Chinese medicine
【DOI】https://doi.org/10.1016/j.jep.2022.115699
【影響因子(IF)】5.19
【出版期刊】《Journal of Ethnopharmacology》
【產(chǎn)品原文引用】
The whole buccal and palatal periodontal tissues of the upper molars were collected and homogenized in PBS. The samples were centrifuged for 15 min at 5000 rpm and 4 ?C. The supernatants were stored at ? 80 ?C for further analysis to evaluate the levels of interleukin 1β (IL-1β), IL-6, IL-8, tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2). The cytokine levels were measured by ELISA assay following the manufacturer’s instructions. The absorbance was measured at 450 nm. Results were calculated using the standard curves created in each assay and shown as ng/L.