恒遠(yuǎn)產(chǎn)品文獻(xiàn):魚維生素K1（VK1）ELISA試劑盒引用文獻(xiàn)
發(fā)布時間:2021/1/18 11:35:07 閱讀次數(shù)：757

【文獻(xiàn)標(biāo)題】Disruption of Abcc6 Transporter in Zebrafish Causes Ocular Calcification and Cardiac Fibrosis

【作者】Jianjian Sun，Peilu She，Xu Liu，et.al

【作者單位】華東師范大學(xué)（East China Normal University）

【文獻(xiàn)中引用產(chǎn)品】

魚維生素K1（VK1）ELISA試劑盒

【關(guān)鍵詞】PXE，abcc6，vitamin K，ocular calcification，cardiac fibrosis

【DOI】https://doi.org/10.3390/ijms22010278

【影響因子(IF)】4.55

【出版期刊】《INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES》

【產(chǎn)品原文引用】

4.12. ELISA Assay

This kit used the double antibody sandwich method to determine the level of vitamin K1 (VK1) in fish (Shanghai Hengyuan Biological Technology Co., Ltd., H-42702, Shanghai,China). The monoclonal antibody vitamin K1 was added into the micropore and combined with horseradish peroxidase (HRP) to form a complex. After polyester, tetramethylbenzidine (TMB) was added to develop color. Under TMB catalysis, it was transformed to blue. There was a positive correlation between the color and the fish vitamin K1 in the fish,measured at 450 nm by enzyme-linked immunosorbent assay. The absorbance (OD value)of fish vitamin K1 in the sample was calculated by the standard curve. To determine the cMGP levels, a human matrix gamma carboxyglutamic acid protein (cMGP) detection kit was selected (Shanghai Hengyuan Biological Technology Co., Ltd., H-11621, Shanghai,China). The purified HRP labeled cMGP antibody was coated on the microporous plate to form a complex. After thorough washing, the substrate TMB was added for color rendering.The experiment was repeated three times.